Content Determination of Fumaric Acid in Pleurotm citrinopileatus by HPLC
- VernacularTitle:HPLC法测定金顶侧耳中延胡索酸的含量
- Author:
Jia LIU
;
Huishun PIAO
;
Suyun SHI
;
Shanyu ZHANG
- Publication Type:Journal Article
- Keywords:
Pleurotm citrinopileatus;
Fumaric acid;
HPLC;
Content determination
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for the determination of fumaric acid in Pleurotm citrinopileatus. METHODS: HPLC was performed on Yilite C18 column(250 mm?4.6 mm,5 ?m) with methanol-0.01 mol?L-1 potassium dihydrogen phosphate solution(5 ∶ 95,pH adjusted to 2.5 using phosphoric acid) as mobile phase and detection wavelength of 216 nm. RESULTS: The linear range of fumaric acid was 0.16～1.6 ?g (r=0.999 9). The average recovery was 101.59% (RSD=1.28%,n=6). CONCLUSION: This method is accurate, simple and reproducible for the content determination of fumaric acid in P. citrinopileatus.
