Anti-tumor effectivity of CTL induced by activated B lymphocyte after hepatocellular carcinoma RNA transfection
- VernacularTitle:肿瘤细胞RNA转染活化的B细胞诱导的CTL抗肿瘤效应
- Author:
Yafeng WAN
;
Xingrui LI
;
Jilin YI
- Publication Type:Journal Article
- Keywords:
Liver Neoplasms;
B Lymphocyte;
CD40 Ligand;
CTL;
APC
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antitumor effectivity of special cytotoxic T lymphocytes(CTLs) induced by B lymphocytes in mice.Methods B lymphocytes were collected,isolated and purfied.Cells were initially activated by CD40L and rmIL-4,then cocultivated with T lymphocytes.T lymphocyte proliferation was examined.Total RNA,which was extracted from Hepal-6(a hepatocelluar carcinoma cell line),were transfected into B lymphocytes,as experimental group;while transfected with RNA of mice liver cells,liposimes and 1640 were as control groups,The expression of antigen presenting cell(APC) markers(CD40,CD80 and CD86) and major histocomability complex(MHC) on B cell surface after transfection were deteced.CTL were obtained by stimulating T lymphocytes with transfected B lymphocytes.Hepal-6 was cell-targeted and examined as index of CTL killing activity.The IFN-r secretion of stimulated CTL was quantified.Results T cell proliferation in experimental group had a higher degree than that in RNA control group(P
