Inducing Differentiation Effect of Selenium Chitosan on HL60 Cells
- VernacularTitle:硒化壳聚糖对HL60细胞的诱导分化作用
- Author:
Shouheng DENG
;
Ping CHEN
- Publication Type:Journal Article
- Keywords:
Selenium chitosan;
Differentiation;
HL60 cells;
c-fos;
c-myc
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of selenium chitosan on differentiation of HL60 cells and its relationship with the c-myc and c-fos protein expression of cells. METHODS:HL60 cells were divided into 5 groups,i.e. 25 mg?L-1 selenium chitosan group,50 mg?L-1 selenium chitosan group,100 mg?L-1 selenium chitosan group,blank control group and positive control group (dimethy sulfoxide). The differentiation of HL60 cells was measured by NBT-reduced method after administration and amount of positive cells was detected. FCM was used to examine the c-myc and c-fos protein expression of cells. RESULTS:As compared with blank control group,differentiation effect of selenium chitosan groups were similar to that of positive control group; NBT-reduced positive cells were increased while c-myc protein expression of cells was decreased(P
