Acute Stress and Chronic Stress Change Brain-Derived Neurotrophic Factor (BDNF) and Tyrosine Kinase-Coupled Receptor (TrkB) Expression in Both Young and Aged Rat Hippocampus.
10.3349/ymj.2010.51.5.661
- Author:
Shou Sen SHI
1
;
Shu Hong SHAO
;
Bang Ping YUAN
;
Fang PAN
;
Zun Ling LI
Author Information
1. Department of Medical Psychology, Institute of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong, China.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
BDNF;
chronic stress;
senescence;
behavior;
plasma corticosterone
- MeSH:
Animals;
Blotting, Western;
Brain-Derived Neurotrophic Factor/genetics/*metabolism;
Corticosterone/blood;
*Gene Expression Regulation;
Hippocampus/*metabolism;
Male;
Radioimmunoassay;
Random Allocation;
Rats;
Rats, Wistar;
Receptor, trkB/genetics/*metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Stress, Physiological/genetics/*physiology
- From:Yonsei Medical Journal
2010;51(5):661-671
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The purpose of this study is to explore the dynamic change of brain-derived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression. MATERIALS AND METHODS: By using forced-swimming in 4degrees C cold ice water and 25degrees C warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session. RESULTS: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress. CONCLUSION: The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.
