Effect of Siwu Decoction on Liver Mitochondrial Proteome of Blood Deficiency Model Rats
- VernacularTitle:四物汤对血虚证模型大鼠肝线粒体蛋白质组的影响
- Author:
Wenjun GONG
;
Xingde WO
;
Dezhao LU
;
Huideng DING
;
Yi LI
- Publication Type:Journal Article
- Keywords:
Siwu Decoction (Decoction of four ingredients);
Blood deficiency syndrome;
Liver mitochondria;
Proteome;
TCM therapy
- From:
Journal of Traditional Chinese Medicine
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the mechanism of Siwu Decoction (Decoction of four ingredients) on blood deficiency syndrome.Methods Wistar rats were randomized into 3 groups: model group, treatment group, and normal group, 6 ones in each. Rats of the model group and the treatment group were injected 2% acetylphenylhydrazine (APH) saline subcutaneously on the first, forth and seventh day. From the forth day, the model group was treated with saline by gavage, the treatment group was treated with Siwu Decoction by gavage, and the normal group was treated with saline for 7 days in succession. Then the amount of peripheral blood cells, wet weight of liver and spleen were detected. Liver mitochondrial proteome of all rats were extracted. Proteomic technology was used to measure the regulated proteins by Siwu Decoction.Results In the model group, the rats had decreased general status significantly such as pale, enlarged liver and spleen, lowered red blood cells (RBC) and hemoglobin (HGB), increased white blood cells (WBC) and reticulocyte (RCT) (P
