Effect of Bushen Huoxue Recipe on the Expression of Collagen Type Ⅳ and Laminin of the Rats with Mesangial Proliferative Glomerulonephritis
- VernacularTitle:补肾活血方对系膜增生性肾小球肾炎大鼠Ⅳ型胶原、层粘连蛋白的影响
- Author:
Mianzhi ZHANG
;
Daning ZHANG
;
Song ZHAO
;
Yanqiu ZHANG
;
Shuxia LIU
;
Limin WANG
- Publication Type:Journal Article
- Keywords:
Bushen Huoxue Recipe (Recipe to reinforce the kidney and activate blood);
Mesangial proliferative glomerulonephritis (MsPGN);
Glomerulosclerosis;
Ⅳ-type Collagen;
Laminin
- From:
Journal of Traditional Chinese Medicine
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Bushen Huoxue Recipe (Recipe to reinforce the kidney and activate blood) in the treatment of glomerulosclerosis of the rats with mesangial proliferative glomerulonephritis (MsPGN).Methods Fifty-four SD male rats were randomized into normal group, model group, and treated group with 18 rats in each. The MsPGN models were made by injection of anti-rat-thymocyte antiserum (ATS) through the tail vein. After modeling, the treated group was administered Bushen Huoxue Recipe with a dosage of 5ml/kg, once a day. Six rats from each group were selected in the second, fourth, and eighth week respectively to determine the content of urine protein (Upro), blood urea nitrogen (BUN), and serum creatinine (SCr), and the kidney was cut for pathological observation and immunohistochemical detection of the kidney tissues, the expression of Ⅳ-type collagen (Col-Ⅳ) and laminin (LN).Results The pathological determination showed that, at the same time points, the hyperplasia of mesangial cells in the treated group was significantly relieved and mesangial matrix reduced as compared with the model group. The content of Upro, BUN and SCr at all time points in the treated group was significantly decreased as compared with the model group (P
