Effect of Different Extracting Methods on the Content Determination of Polysaccharide from Caulis Derdrobii
- VernacularTitle:不同提取方法对石斛多糖含量测定的影响
- Author:
Aoxue LUO
;
Yijun FAN
- Publication Type:Journal Article
- Keywords:
Caulis Dendrobii;
Polysaccharide;
Phenol-sulfuric acid method;
Ultrasonic-assisted extraction;
Thermal reflux extraction
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study on the effect of different extracting methods on the content determination of polysaccharide from Caulis Dendrobii.METHODS: Polysaccharide was extracted from Caulis Dendrobii respectively by reflux extraction,improved reflux extraction,ultrasound wave extraction and ultrasonic-assisted thermal reflux extraction.The content of polysaccharide was determined by phenol-sulfuric acid method.RESULTS: The determined results using these methods were as follows:(13.473?0.634)%,(15.692?0.743)%,(11.87?0.536)% and (16.29?0.407)%.CONCLUSION: The content determination of polysaccharide from Caulis Dendrobii was associated with the extraction method.The polysaccharide from Caulis Dendrobii could be extracted more thoroughly and rapidly by ultrasonic-assisted extraction technology.Therefore this method could be used to pretreat the sample in content determination of Caulis Dendrobili polysaccharide.
