Determination of Aminophylline,Eyhedrine and Guaifenesin in Xiaoer Kechuan Oral Solution by RP-HPLC
- VernacularTitle:RP-HPLC法测定小儿咳喘口服液中氨茶碱、麻黄碱和愈创木酚甘油醚的含量
- Author:
Jian CAO
;
Xiaoyuan ZHENG
;
Fang WANG
;
Linqing HUANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Xiaoer kechuan oral solution;
Aminophylline;
Eyhedrine;
Guaifenesin;
Content determination
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an RP-HPLC method for the determination of aminophylline,eyhedrine and guaifenesin in Xiaoer kechuan oral solution.METHODS: The determination was performed on Hypersil ODS column(150 mm?4.6 mm,5 ?m).The mobile phase consisted of acetonitrile-0.015 mol?L-1 KH2PO4(0.1% triethylamine and pH value of phosphoric acid adjusted to 2.7)(10 ∶ 90) with a flow rate of 1.6 mL?min-1 and detection wavelength of 254 nm and 209 nm.RESULTS: The linear ranges were 356.4～712.8 ?g?mL-1 for aminophylline,88～176 ?g?mL-1 for eyhedrine and 106～212 ?g?mL-1 for guaifenesin(r=0.999 9).The average recovery rates were 101.0%(RSD=0.73%,n=9),99.7%(RSD=1.00%,n=9),98.3%(RSD=0.44%,n=9),respectively.CONCLUSIONS:The method is simple and accurate for quality control of Xiaoer kechuan oral solution.
