Determination of Aesculin and Aesculetin in Jiuxiang Zhixie Tablets by HPLC
- VernacularTitle:HPLC法测定九香止泻片中秦皮甲素和秦皮乙素的含量
- Author:
Biqing ZHAO
;
Qun HE
;
Jiuxiang TENG
;
Zhipei PENG
;
Hui LI
;
Shi WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Jiuxiang zhixie tablet;
Content determination;
Aesculin;
Aesculetin
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for the determination of aesculin and aesculetin in Jiuxiang zhixie tablets.METHODS: HPLC method was developed to quantitative determination.Thermo C18(250 mm?4.60 mm,5?m) column was adopted.The mobile phase consisted of acetonitrile-0.1%H3PO4(12 ∶ 88)with flow rate of 1.0 mL?min-1 and the detection wavelength of 334 nm.The column tempreture was set at 30℃.RESULTS: The linear range of aesculin was 80～800 ng(r=0.999 8).The average recovery was 100.1%(RSD=1.89%,n=9).The linear range of aesculetin was 32.96～329.6 ng(r=0.999 5).The average recovery of aesculetin was 101.5%(RSD=2.42%,n=9).CONCLUSION: The method is simple,accurate for the content determination of Jiuxiang zhixie tablet.
