Isolation and Identification of Coumarin in Dendrobium denneanum Cultivated in Sichuan and Its Quality Standard Study
- VernacularTitle:川产迭鞘石斛中香豆素的分离鉴定及药材质量标准研究
- Author:
Min LI
;
Youping LIU
;
Jingxian FAN
;
Ling ZHANG
;
Huiyong LI
- Publication Type:Journal Article
- Keywords:
Dendrobium denneanum;
Coumarin;
Quality criteria
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality creteria of Dendrobium denneanum cultivated in Sichuan.METHODS: The reference substance had been isolated and identified.HPLC and TLC were applied for determination and identification respectively.RESULTS: The coumarin control had been successfully isolated and the TLC spots were clear.The linear range of coumarin was 0.096~0.480 ?g with an average recovery of 99.1%(RSD=1.44%,n=6).CONCLUSION: The results can overall reflect the internal quality of D.denneanum cultivated in Sichuan and provide basis for quality evaluation and development of D.denneanum from Sichuan.