Experimental Study on Apoptosis of Human Gastric Carcinoma SGC-7901 Cells Induced by Ethanol Extracts of Solanum lyratum
- VernacularTitle:白英乙醇提取物诱导人胃癌SGC-7901细胞凋亡的实验研究
- Author:
Guoying TENG
;
Jian WU
;
Xiaolong LIU
- Publication Type:Journal Article
- Keywords:
Solanum lyratum;
Ethanol extracts;
Gastric carcinoma SGC-7901;
Apoptosis;
Gene expression
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To explore the effect of ethanol extracts of Solanum lyratum on apoptosis of SGC-7901 cells and the mRNA expression of apoptosis associated genes Fas and caspase-3.METHODS:SGC-7901 Cells were cultured in vitro and randomly divided into the contral group,ethanol extracts of S.lyratum treatment groups(2.5 mg?mL-1,5 mg?mL-1,10 mg?mL-1) and cisplatin group.The proliferation inhibitory rate was evaluated by MTT assay.Morphological changes and apoptosis of SGC7901 cells were observed under fluorescence microscope.The mRNA expression of Fas gene and caspase-3 were detected by semi-quantitive RT-PCR method.RESULTS: As compared with control group,the cell proliferation inhibitory rate and apoptosis rate of SGC-7901 cells were increased obviously in S.lyratum treatment groups(P
