The expression and significance of FHIT gene in cultured cholangiocarcinoma cells
- VernacularTitle:体外培养胆管癌细胞中FHIT基因的表达及其意义
- Author:
Fang XIE
;
Qiang HUANG
- Publication Type:Journal Article
- Keywords:
Bile Duct Neoplasms;
Genes,Fragile Hislindine Triad;
Gene Expression
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of fragile histidine triad(FHIT) gene in the cholangiocarcinoma cell line QBC939.Methods The eukaryotic expression vector was transfected into QBC939,real-time RT-PCR was used to detect the change of FHIT expression,and direct counting method under microscope was used to detect the change in the biological behavior of cells.Results After transfection,the expression of FHIT mRNA increased by 2.53-fold,but the cell clones were significantly less [(11.2?1.3) vs.(10.5?1.1) vs.(6.3?1.0),P
