Effect of CO_2 pneumoperitoneum on expressions of survivin in gallbladder carcinoma cell line
- VernacularTitle:CO_2气腹对胆囊癌细胞survivin表达的影响
- Author:
Bin ZHANG
;
Hanbin SHEN
;
Shuping ZHENG
- Publication Type:Journal Article
- Keywords:
Carbon Dioxide;
Pneumoperitoneum;
Gallbladder Neoplasms;
Survivin
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of CO2 pneumoperitoneum on the anti-apoptotic capability of gallbladder carcinoma cell line (GBC-SD).Methods The CO2 pneumoperitoneum was established in vitro.After GBC-SD cells were exposed to CO2 pneumoperitoneum at 12 mmHg for 1,2,3,4 h,the expressions of survivin mRNA was examined by RT-PCR.The untreated GBC-SD cells served as control.Results Under a pressure of 12 mmHg,the expressions of survivn had an increasing tendency with the exposure time.Conclusions Under certain pressure and within certain exposure time,CO2 pneumoperitoneum could improve anti-apoptotic capability of GBC-SD cells,which may be one of the mechanisms of the promoting effect of carbon dioxide pneumoperitoneum on malignant cell growth.
