Expression of VEGF mRNA induced by angiotensinⅡ in human hepatic cancer cell line
- VernacularTitle:血管紧张素Ⅱ诱导VEGFmRNA在人肝癌细胞中的表达
- Author:
Jian ZHANG
;
Xue XING
;
Hongguang HAN
;
Zhong GE
- Publication Type:Journal Article
- Keywords:
Carcinoma,Hepatocellular;
AngiotensinⅡ;
Vascular Endothelial Growth Factor A;
Cell Proliferation
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of angiotensin Ⅱ (Ang Ⅱ) on the expression of vascular endothelial growth factor (VEGF) in human hepatic cancer cell line Hep G2.Methods Cultured Hep G2 cells were treated by AngⅡ with various concentrations and were collected at different time points.Then total RNA was extracted.The expression of VEGF mRNA in cultured Hep G2 was determined by relative quantitative reverse transcription polymerase chain reaction(RT-PCR),and the proliferation was detected by methyl thiazolyl tetrazolium (MTT).Results AngⅡ stimulated the proliferation of HepG2 cells,and enhanced the expression of VEGF mRNA (P
