Determination of Serum Concentration of Ganciclovir in Dogs and Study on Relative Bioavailability of Ganciclovir Dispersible Tablets
- VernacularTitle:更昔洛韦在犬体内血药浓度的测定及其分散片的相对生物利用度研究
- Author:
Wei WU
;
Desheng MENG
- Publication Type:Journal Article
- Keywords:
HPLC;
Ganciclovir;
Dogs;
Dispersible tablets;
Capsule;
Serum concentration;
Pharmacokinetics;
Relative bioavailability
- From:
China Pharmacy
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for determination of ganciclovir in serum of dogs and to study pharmacokinetics and relative bioavailability of Ganciclovir dispersible tablets.METHODS:The determination was performed on Dikma DiamonsilTM C18 column.The mobile phase consisted of 0.015 mol?mL-1 monobasic potassium phosphate buffer solution (pH 2.50,containing 0.25% acetonitrile) with flow rate of 1.75 mL?min-1 at detection wavelength of 254 nm.The column temperature was set at 40 ℃.In a randomized cross-over study,a single dose of dispersible tablets and the capsule were given to 6 dogs.An HPLC method was used to determine plasma concentration of ganciclovir in dogs and the pharmacokinetic data were treated by DASver1.0 software.RESULTS:The linear range of ganciclovir were 0.050 25～100.5 ?g?mL-1;the methodological recovery was larger than 90% (RSD
