Identification of Descurainia sophia and Plantago asiatica by HPCE and PCR Assay
- VernacularTitle:高效毛细管电泳结合PCR法鉴别南葶苈子与车前子
- Author:
Dan GUO
- Publication Type:Journal Article
- Keywords:
Descurainia sophia;
Plantago asiatica;
HPCE;
PCR;
Identification
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPCE and polymerase chain reaction (PCR) assay for identification of Descurainia sophia and Plantago asiatica.METHODS: PCR products of D. sophia and P.asiatica were analyzed by capillary tube (60 cm?75 ?m), taking 30 mmol?L-1 sodium tetraborate decahydrate (pH 9.2) as run buffer. The injection sample was performed by high pressure of 5 s at a separation voltage of 12 kV and with UV detection wavelength of 254 nm. RESULTS: PCR products of D. sophia and P. asiatica were separated by HPCE rapidly and accurately. The significant differences were noted in electrophoregram between PCR products of D. sophia and P. asiatica.CONCLUSION: This method is simple, rapid and sensitive for identification of D.sophia and P. asiatica.
