C/EBP? is involved in transcriptional regulation of mVGLUT2 gene expression
- VernacularTitle:C/EBP?参与小鼠胰岛?细胞囊泡谷氨酰胺转运子2基因表达的调控
- Author:
Zhuguo WU
;
Shiming LIU
;
Xinchun CHEN
- Publication Type:Journal Article
- Keywords:
Mouse pancreatic ? cells;
Promoter;
Gene expression regulation;
CCAAT-enhancer-binding protein ?;
Vesicular glutamate transporter 2
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:The main purpose of this study is to investigate the regulatory role of C/EBP? in mouse vesicular glutamate transporter 2(mVGLUT2) gene expression. METHODS:The promoter region of mVGLUT2 was cloned to PGL3-basic vector. Site-direction mutation was used to identify the CCAAT-enhancer-binding protein ?(C/EBP?) binding site. The promoter activity was observed by luciferase system. The binding between C/EBP? protein and mVGLTU2 promoter region was determined by EMSA. The C/EBP? gene expression was inhibited by its specific siRNA. RESULTS:mVGLUT2 promoter activity decreased about 50% after mutation of C/EBP? binding site. EMSA showed that C/EBP? protein bound onto mVGLUT2 promoter region. Meanwhile,when C/EBP? gene expression was inhibited by its specific siRNA,mVGLUT2 promoter activity,mRNA level and protein level were decreased about 60%,40% and 45%,respectively. CONCLUSION:C/EBP? is involved in the regulation of mVGLUT2 gene expression.
