Poly adenosine diphosphate-ribose polymerase regulates the expression of nuclear factor-?B and related inflammatory factors in rat hippocampus after epilepsy
- VernacularTitle:PARP调节癫痫大鼠海马核转录因子?B及相关炎症因子的表达
- Author:
Shengjun WANG
;
Zhaofu CHI
;
Shuhua WANG
;
Lingyi CHI
;
Xiuhe ZHAO
- Publication Type:Journal Article
- Keywords:
Epilepsy;
Poly adenosine diphosphate-ribose polymerase;
NF-kappa B;
Interleukin-1;
Cyclooxygenase-2
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the time course of nuclear factor-?B (NF-?B) and the effects of 3-aminobenzamide (3-AB) on the expressions of NF-?B,interleukin-1? (IL-1?) and cyclooxygenase-2 (COX-2) in hippocampus after seizures. METHODS:Epilepsy were induced by kainic acid through cerebral ventricular injection. Western blotting was used to detect NF-?B p65 expression in nucleus at various experiment groups. Moreover,mRNA and protein expressions of IL-1? and COX-2 in different experiment groups were determined by RT-PCR and Western blotting analysis. RESULTS:NF-?B p65 immunoreactivity began to increase in the nuclear fraction at 2 h (P
