Determination of Nimesulide in Human Plasma by LC-MS
- VernacularTitle:LC-MS法测定人血浆中尼美舒利的浓度
- Author:
Wei LIU
- Publication Type:Journal Article
- Keywords:
LC-MS;
Nimesulide;
Plasma concentration;
Pharmacokinetics
- From:
China Pharmacy
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an LC-MS method for the determination of nimesulide in human plasma. METHODS:The separation was performed on Agilent Zorbox SB-Cl8 column,using acetonitrile-0.1% formic acid (65 ∶ 35) as the mobile phase at a flow rate of 1.0 mL?min-1. The column temperature was set at 35 ℃ and valsartan was served as internal standard. Positive electrospray ionization detection was applied to monitor m/z 309(nimesulide,[M+H]+)and m/z 436(valsartan,[M+H]+). RESULTS:The linear range of nimesulide was 5～50 ng?mL-1(r=0.998 9). The method recovery of nimesulide ranged from 87.1% to 92.9%. The intra-day and inter-day RSD were 3.1%～5.8%,6.4%～9.2%,respectively.CONCLUSION:The method is sensitive,simple,convenient,accurate and reproducible,and it is suitable for clinical plasma concentration monitoring and pharmacokinetics research of nimesulide.
