Study on the Effect of Naringin on Chemokine RANTES Secretion in Human Keratinocyte and NF-?B Signal Pathway
- VernacularTitle:柚皮苷对人皮肤角质细胞分泌趋化因子RANTES及其核转录因子-?B信号通路影响的实验研究
- Author:
Liao LIU
;
Ping JIA
- Publication Type:Journal Article
- Keywords:
TNF-?;
IFN-?;
RANTES;
Naringin;
HaCaT cells;
NF-?B
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of inflammatory cytokine TNF-? and IFN-? on RANTES secretion in human kera-tinocyte(HaCaT)and the inhibition effect of naringin on induction mechanism by using acitretin as positive control.METHODS:The HaCaT cells were assigned into negative control group,model stimulation group(treated with TNF-?+IFN-?),acitretin pretreatment group(treated with TNF-?+IFN-?+acitretin),naringin pretreatment group(treated with TNF-?+IFN-?+naringin).The concentration of RANTES in HaCaT cells which were stimulated by cytokine TNF-? and IFN-? alone or together after acitretin and naringin were added into stimulators was determined by ELISA assay.The expressions of NF-?B P65 protein in HaCaT cells was detected with immunocytochemical method and Western blot method.RESULTS:The concenctration of RANTES in HaCaT cells which were stimulated by cytokine TNF-? or IFN-? alone or together increased;the greatest concentration was observed in model stimulation group(P
