Regulating Effect of Nitrogen Monoxide on Gene Expression of Sodium/Iodide Symporter in Human Thyrocyte
- VernacularTitle:一氧化氮对人甲状腺细胞钠/碘转运体基因的调节作用
- Author:
Xinyu LIU
- Publication Type:Journal Article
- Keywords:
Nitrogen monoxide;
Sodium nitroprusside;
Thyrocyte;
Sodium/iodide symporter;
Expression
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the influence of nitrogen monoxide(NO) donor,sodium nitroprusside(SNP),on mRNA expression of sodium/iodide symporter(NIS) in thyrocyte membrane cultured in vitro in order to interpret molecular mechanism that NO inhibits the uptake of iodide.METHODS:The effect of SNP at different concentrations(0,10-6,10-5,10-4,10-3 mmol?L-1) on mRNA expression of NIS in thyrocyte by using cell culture method and RT-PCR with optical density ratio of NIS/?-actin as index.RESULTS:Optical density ratio of NIS/?-actin in each group decreased in order after treated with SNP(0,10-6,10-5,10-4,10-3 mmol?L-1).NO inhibited mRNA expression of NIS in a dose-dependent manner(P
