Determination of the Content of Glucose in Glucose/Sodium Chloride Injection by HPLC
- VernacularTitle:HPLC法测定葡萄糖氯化钠注射液中葡萄糖的含量
- Author:
Gengshen SONG
;
Jianguo JIANG
;
Ting SUN
- Publication Type:Journal Article
- Keywords:
HPLC;
Glucose/Sodium Chloride injection;
Glucose;
Content determination
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of the content of glucose in Glucose/Sodium Chloride injection.METHODS:The determination was performed on Waters Sugar PakⅠ column with column temperature set at 80 ℃,water as mobile phase and injection volume of 20 ?L.The cell temperature of RID detector was set at 40 ℃.RESULTS:The linear range of glucose was 0.05～2.50 mg?mL-1(r=0.999 9) with the lowest detection limit of 24 ng.The average recovery ranged from 99.80% to 99.90%(RSD=0.21%).CONCLUSION:The method is simple,rapid,accurate,sensitive and reliable in operation,and it is applicable for determination of glucose in Glucose/Sodium Chloride injection.
