Determination of Eptifibatide and Related Peptide in Eptifibatide Injection by HPLC
- VernacularTitle:HPLC法测定依替巴肽注射液中主药及杂质相关肽的含量
- Author:
Li YAO
;
Chunlai LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Eptifibatide;
Related peptide;
Content determination
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for determination of major component and related peptide in eptifibatide injection.METHODS:The determination was performed on AichromBond-AQ C18 column with column temperature at 25 ℃ and flow rate of 1.0 mL?min-1.The mobile phase consisted of acetonitrile-water(0.13% trifluoroacetic acid).The detection wavelength was set at 220 nm and injection volume was 20 ?L.RESULTS:The linear range was 75.2～1 203.6 ?g?mL-1(r=0.9999) with mean recovery of 99.73%～99.95% (RSD=0.26%).CONCLUSION:The method is simple and rapid and it is applicable for determination of major component and related peptide in eptifibatide injection.
