Optimization of Cellulase-assited Extraction Technology of the Steam-processed Scutellaria baicalensis by Orthogonal Experiment
- VernacularTitle:正交试验优选蒸制黄芩的纤维素酶辅助提取工艺
- Author:
Chunhao YU
- Publication Type:Journal Article
- Keywords:
Scutellaria baicalensis;
Steam-processed;
Baicalin;
Wogonoside;
Cellulase;
Assisted extraction;
Orthogonal experiment
- From:
China Pharmacy
2007;0(36):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTVIE:To optimize the cellulase-assisted extraction technology of steam-processed Scutellaria baicalensis. METHODS:The cellulase-assisted extraction technology of steam-processed S.baicalensis was optimized by orthogonal experiment with the extraction rates of baicalin and wogonoside as indexes and with cellulase concentration,extracting temperature,extracting time and pH value as factors. RESLUTS:The optimal cellulase-assisted extraction technology of steam-processed S.baicalensis was as follows:the concentration of cellulase was 15 U?g-1; the extracting temperature was 50 ℃; the extracting time was 7 h and the pH value was 4.8. Under the optimal conditions,the extraction rates of baicalin and wogonoside were 8.44% and 2.62%,respectively. CONCLUSION:The cellulase-assisted extraction technology of steam-processed S.baicalensis is superior to the traditional water-decoction method,and it proves to be an effective process for the extraction of baicalin and wogonoside from steam-processed S.baicalensis and by which the utilization rate of S.baicalensis can be improved.
