Simultaneous Determination of the Contents of Costunolide and Dehydrocostuslactone in Xuedan Weichang Pill by RP-HPLC
- VernacularTitle:RP-HPLC法同时测定雪胆胃肠丸中木香烃内酯和去氢木香内酯的含量
- Author:
Zhaorong GUO
;
Shilin YANG
;
Xiaoran LI
;
Lili HAO
;
Qiongming XU
;
Shaohua CHEN
- Publication Type:Journal Article
- Keywords:
Xuedan weichang pill;
Costunolide;
Dehydrocostuslactone;
RP-HPLC;
Content determination
- From:
China Pharmacy
2007;0(36):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an RP-HPLC method for simultaneous determination of the contents of costunolide and dehydrocostuslactone in Xuedan weichang pill. METHODS:The determination was performed on Shim-pack VP-ODS column(150 mm?4.6 mm,5 ?m) with methanol-water(60∶40) as mobile phase and the detection wavelength was set at 225 nm.RESULTS:The linear ranges of costunolide and dehydrocostuslactone were 13.6～272.0 ?g?mL-1 and 11.25～225.0 ?g?mL-1,respectively,and their average recoveries were 99.47% and 99.62%,respectively with RSD of 1.75%(n=6) and 1.14%(n=6),respectively. CONCLUSION:The method established is simple,feasible,accurate and reliable,and it is applicable for the quality control of Xuedan weichang pill.
