Determination of Oleic Acid and Linoleic Acid in Brucea javanica Oil by RP-HPLC
- VernacularTitle:RP-HPLC法测定鸦胆子油中油酸和亚油酸的含量
- Author:
Ying LI
;
Qi SHEN
;
Ming YIN
- Publication Type:Journal Article
- Keywords:
Brucea javanica oil;
Pre-column derivatization;
RP-HPLC;
Oleinic acid;
Linolic acid;
Content determination
- From:
China Pharmacy
2007;0(36):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an RP-HPLC method for determination of the contents of oleinic acid and linolic acid in Brucea javanica oil. METHODS:The derivative fatty acid was obtained from extracting and separating process using ?-bromo-acetophenone as derivative reagent,K2CO3 as base and N,N-dimethylformamide (DMF) as solvent. The sample was separated on Shim-pack VP-ODS column(150 mm?4.6 mm,5 ?m); the detection wavelength was set at 242 nm. The mobile phase consisted of methanol-acetonitrile-water(30∶20∶50). RESULTS:The linear ranges of oleinic acid and linolic acid were 0.025～1.0 mg?mL-1(r=0.999 6) and 0.1 ～ 0.8 mg?mL-1(r=0.999 0),respectively. The average recoveries were 98.54% and 98.50%,respectively,and their RSD were 1.81% and 1.90%,respectively (n=6 each). CONCLUSION:The method is accurate and specific,and it is applicable for the quality control of B.javanica oil.
