Analysis and Determination of the Composition of Monosaccharide in Polysaccharide from Leaves and Twigs of Morus nigra from Xinjiang
- VernacularTitle:新疆药桑叶、枝多糖中单糖的组成分析与测定
- Author:
Lian SUN
;
Lei MENG
;
Yan WANG
;
Xuan ZHANG
- Publication Type:Journal Article
- Keywords:
Morus nigra from Xinjiang;
Mulberry leaves;
Mulberry twig;
Polysaccharides;
Monosaccharide;
RP-HPLC;
Pre-column derivatization
- From:
China Pharmacy
2007;0(36):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze and determine the compositions of monosaccharide in polysaccharide from leaves and twigs of Morus nigra from Xinjiang.METHODS:The polysaccharides in leaves and twigs of M.nigra were extracted by water and precipitated by ethanol then hydrolyzed into monosaccharide by using 2 mol?L-1 H2SO4.The monosaccharide was derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP) and determined by RP-HPLC. RESULTS:The polysaccharide in leaves and twigs of M.nigra were composed of mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose and arabinose etc. All the 7 monosaccharides were well-separated within 25 min. CONCLUSION:The method is simple and sensitive with accurate results,and it can be used for the quality control of leaves and twigs of M.nigra from Xinjiang.
