The expression of TLR4 signaling pathway in rat with secretory otitis media
- VernacularTitle:Toll样受体4信号途径在大鼠中耳积液中的表达
- Author:
Tao FU
;
Ping WANG
;
Baodong DU
;
Wei ZHU
- Publication Type:Journal Article
- Keywords:
Rats;
Otitis Media with Effusion;
Animal Laboratory
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the role of TLR4 signaling pathway in secretory otitis media of rats.METHODS Rats SOM models were made by infusing FS and LPS to the middle otocysts. The rats were killed separately at the 3rd, 5th, 7th, 9th,11th and13th day(5 rats each time). And then the expressions of TLR4, iNOS, IL-8mRNA of middle ear mucosae were detected. NF-?B was also detected through the method of immunohistochemistry. RESULTS The expressions of TLR4mRNA, iNOS and IL-8mRNA in middle ear mucosae of SOM group were significantly higher than that of control group. Immunohistochemistry method showed that the activation of NF-?B in middle ear mucosae was obviously increased in SOM. CONCLUSION The expression of TLR4 was obviously increased in SOM and it activates the NF-?B, promotes the expression of downstream IL-8. That maybe the mechanism which cause SOM.
