Determination of Concentration of Netilmicin Sulfate in Human Serum by Ion-pair RP-HPLC
- VernacularTitle:离子对RP-HPLC法测定人血清中硫酸奈替米星的浓度
- Author:
Hongwen WU
;
Yazhou WANG
;
Jinsheng LI
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Ion-pair;
Netilmicin sulfate;
Serum drug concentration
- From:
China Pharmacy
2007;0(35):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an ion-pair RP-HPLC method for determination of the concentration of netilmicin sulfate in human serum.METHODS:The determination was performed on Luna C18 column with column temperature set at 40℃;the mobile phase consisted of acetonitrile-water (15∶85,pH 2.0,containing 10 mmol?L-1 sodium heptanesulfonate) at a flow rate of 0.8 mL?min-1.The detection wavelength was set at 205 nm.RESULTS:The linear range of netilmicin was 2～20 mg?L-1(r=0.997 6) with a recovery rate of 93.66%～96.05%.The intra-day and inter-day RSD were both less than 10%,respectively.CONCLUSION:The method is sensitive and simple with accurate and reliable results,and it is able to satisfy the requirement for the determination of serum concentration of netilmicin.
