PPAR-? involves in cardiomyocyte hypertrophy induced by high glucose and insulin
- VernacularTitle:PPAR-?参与高糖高胰岛素诱导的心肌细胞肥大
- Author:
Mingfeng WANG
;
Qingsong JIANG
;
Qin WU
;
Xiaoyan WU
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferator-activated receptor-?;
Cyclooxygenase-2;
Myocardial hypertrophy;
High glucose and high insulin
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the role of peroxisome proliferator-activated receptor-? (PPAR-?) signal transduction pathway in cardiac hypertrophy induced by high glucose and insulin (HGI). METHODS:The cultured neonatal rat cardiomyocytes were used to observe the effect of fenofibrate (FF),a selective PPAR-? agonist,on cardiomyocyte hypertrophy induced by HGI (glucose at concentration of 25.5 mmol/L and insulin at 0.1 ?mol/L). The cardiomyocyte hypertrophic responses were assayed by measuring the cell surface area,protein content,and mRNA expression of atrial natriuretic factor (ANF). The expressions of mRNA and protein were assayed by real -time PCR and Western blotting. RESULTS:In cultured cardiomyocytes,HGI induced profound change of hypertrophic morphology,the significant increase in cell surface area,protein content and ANF mRNA expression compared to those in vehicle control (P
