Angiotensin II-induced matrix metalloproteinase-9 expression mediated by NF-?B pathway in human THP-1 cells
- VernacularTitle:NF-?B信号通路介导AngⅡ诱导THP-1巨噬细胞表达MMP-9
- Author:
Chanjuan CHAI
;
Zhiming YANG
;
Yuming KANG
;
Chuanshi XIAO
- Publication Type:Journal Article
- Keywords:
Angiotensin Ⅱ;
NF-kappa B;
Matrix metalloproteinase-9;
THP-1 cells
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:The present study was undertaken to investigate the effect of angiotensin II (AngⅡ) on expression of MMP-9 in THP-1 macrophages. METHODS:Macrophages converted from THP-1 monocytes by incubating with PMA (0.1 ?mol/L) for 48 h were divided into PMA group; PMA+AngⅡ group (10-7mol/L,1 h); PMA+AngⅡ+PDTC group (10 ?mol/L,30 min) and PDTC group. Western blotting was used to detect the MMP-9 and phosphorylation of NF-?B p65,and the expression of MMP-9 mRNA in THP-1 macrophages was measured by RT-PCR.RESULTS:Compared to control group,the expression of MMP-9 (1.06?0.11,P
