Effect of NF-?B inhibitor pyrrolidine dithiocarbonate on the proliferation and apoptosis in K562 cells
- VernacularTitle:NF-?B抑制剂PDTC抗白血病细胞增殖的实验研究
- Author:
Chun ZHANG
;
Guohui CUI
;
Fang LIU
- Publication Type:Journal Article
- Keywords:
NF-?B inhibitor;
Cell proliferation;
Apoptosis;
K562 cells;
Pyrrolidine dithiocarbamate
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of pyrrolidine dithiocarbamate (PDTC),a specific inhibitor of NF-?B on the proliferation and apoptosis of K562 cells and to explore the anti-tumor mechanism of PDTC.METHODS:Trans AMTM NF-?B p65 kit was used to detect the activity of p65 in K562 cells treated by PDTC. The effect of PDTC on the proliferation of K562 cells was measured by WST-1 method. DNA damage was detected by single cell gel electrophoresis (comet assay). The procaspase-3 and activated protein level of caspase-3 were detected by Western blotting.RESULTS:The activity of p65 in K562 cells was inhibited after treated by PDTC (P
