Determination of Bortezomib in Its Crude Drug by RP-HPLC
- VernacularTitle:RP-HPLC法测定硼替佐米原料药中主药的含量
- Author:
Jiagen XU
;
Kangkang XU
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Bortezomib;
Content determination
- From:
China Pharmacy
2007;0(34):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an RP-HPLC method for determination of the content of bortezomib in its crude drug. METHODS: Bortezomib was separated on Phenomenex-C18 chromatographic column with mobile phase consisted of methanol-phosphate buffer (pH 2.0) (60∶40) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm. RESULTS: The linear range of bortezomib was 50.72～152.16 ?g (r=0.999 9) with an average recovery rate of 99.62% (RSD=0.39%,n=6). CONCLUSIONS: The method is sensitive,specific and simple in operation,and it is applicable for the determination of bortezomib in its crude drug.
