Determination of Total Flavonoids and Quercetrin in Different Parts of Apocynum venetum from Songnen Grassland & Its Antioxidant Activity
- VernacularTitle:松嫩草原罗布麻各器官总黄酮和槲皮素的含量测定及药材抗氧化活性评价
- Author:
Qinghua LI
- Publication Type:Journal Article
- Keywords:
Apocynum venetum;
Songnen grassland;
Total flavonoids;
Quercetin;
Content;
Antioxidant activity
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determine the contents of total flavonoids and quercetin in different parts of Apocynum venetum collected in Songnen grassland and study their antioxidant activities so as to provide references for efficient utilization of the herbal plant.METHODS: Ultrasonic extraction was applied to extract total flavonoids.The content of total flavonoids was determined by chromatometry and the content of quercetin was determined by HPLC.The antioxidant activities of the extracts from different parts of A.venetum were evaluated by determining their abilities in scavenging 1,1-diphenyl-2-picrylhydrazyl(DPPH?) radicals and?OH radicals.RESULTS: The contents of quercetin and total flavonoids were richer in both leaves and flowers of A.venetum than in stems.In addition,the total flavonoids extracted from the leaves and flowers of A.venetum showed higher antioxidant activity than from the stems.CONCLUSION: The leaves,flowers and stems of A.venetum can all be used as raw materials of natural antioxidants.