The Effect of the Glutamate/Aspartate Transporter Antibody on Auditory Brainstem Response and Morphology of Hair Cells in Guinea Pig's Cochlea
- VernacularTitle:谷氨酸/天冬氨酸转运体抗体对豚鼠听性脑干反应及毛细胞形态的影响
- Author:
Yubin JI
;
Jinlin WANG
;
Jianhua QIU
;
Shunli LIU
- Publication Type:Journal Article
- Keywords:
Glutamate/aspartate transporters(GLAST);
Cochlea;
Guinea pig
- From:
Journal of Audiology and Speech Pathology
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this investigation was to observe the effects of the glutamate/aspartate transporter(GLAST) antibody to auditory brainstem response(ABR) and the pathologic morphology of hair cells in the guinea pig's cochlea. Methods 20 guinea pigs were randomly divided into the experimental group and control group. By perfusing the antibody to GLAST into tympanic canal in the cochlea of guinea pigs in experimental group and artificial perilymph into the guinea's cochlea in control group,the results of ABR ,basilar membrane stretched preparation and transmission electron microscope were observed. Results After antibody perfusion,the ABR was not induced from the third day to the ninth day.In control group,the ABR was nearly absent on the third day,but could be evolved,on the sixth day and the ninth day in all guinea pigs with the average threshold of 62.50?5.25 dB SPL and 47.50?6.18 dB SPL,respectively. The ABR thresholds at different time in control group were significantly different(P
