Titer Determination of Main Component in Kitasamycin Tablets and Acetylkitasamycin Dry Suspension by Turbidimetry
- VernacularTitle:浊度法测定吉他霉素片与乙酰吉他霉素干混悬剂中主药的效价
- Author:
Yanxia GAO
;
Jianguo JIANG
;
Bin HAN
- Publication Type:Journal Article
- Keywords:
Turbidimetry;
Kitasamycin tablets;
Acetylkitasamycin dry suspension;
Titer
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the titer of main component in kitasamycin tablets and acetylkitasamycin dry suspension.METHODS:Staphylococcus aureus was used as test organism and which were cultured at (37?0.5) ℃ for about 4～5 hours by adding 1.0%～1.5% kitasamycin and 2.0%～2.5% acetylkitasamycin,then the absorbance was determined at a wavelength of 530 nm.RESULTS:The linear range of kitasamycin was 0.5～4.0 IU?mL-1 and that of acetylkitasamycin was 0.45～6.3 IU?mL-1,and their recovery rates were 101.7%(RSD=1.9%,n=9) and 101.1%(RSD=2.1%,n=9),respectively.CONCLUSION:The established turbidimetry is sensitive,rapid yet with few influencing factors,thus it is applicable for the titer determination of main component in kitasamycin tablets and acetylkitasamycin dry suspension.
