Determination of Optical Purity of Lactic Acid by HPLC-CSP
- VernacularTitle:HPLC手性固定相法测定乳酸光学纯度
- Author:
Quanyi WANG
;
Yiwen ZHANG
;
Dan YANG
;
Yumei WANG
;
Hang SONG
- Publication Type:Journal Article
- Keywords:
HPLC;
Chiral stationary phase;
Lactic acid;
Enantiomers;
Optical purity
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC-chiral stationary phase(CSP) for separating the enantiomers of lactic acid and determining the optical purity of lactic acid.METHODS:Lactic acid samples of different optical purity were determined by HPLC-CSP vs.optical rotation method.The enantiomers of lactic acid were separated on Chirex 3126(D)-penicillamine chiral column.The mobile phase consisted of copper sulfate solution-isopropyl alcohol (85∶15) at a flow rate of 0.6 mL?min-1.The detection wavelength was set at 254 nm.RESULTS:A baseline separation of the enantiomers of lactic acid was achieved with a separation factor of greater than 1.2.The calibration curve was linear in the range of 0.75～48.24 mg?mL-1 (r=0.999 3),RSD=1.21%.The determination results determined by HPLC-CSP were similar to those determined by optical rotation method.CONCLUSION:The established method is simple,reliable in results and reproducible,and it is applicable for the separation of the enantiomers of lactic acid and determination of the optical purity of lactic acid.
