Separation and Purification of the Ethanol Extracts of Polygonum multiflorum and Study on Its Activity
- VernacularTitle:何首乌醇提物的分离纯化及活性研究
- Author:
Shiqian HUANG
- Publication Type:Journal Article
- Keywords:
Polygonum multiflorum;
Ethanol extract;
Separation;
Purification;
Human umbilical vein vascular endothelial cell(HUVEC)
- From:
China Pharmacy
2007;0(30):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To separate and purify the effective parts of the extracts of Polygonum multiflorum and study the activity of the effective parts.METHODS:The n-butanol extracts of P.multiflorum extracts were separated by silica gel column chromatography and eluted with different concentration of mixture of chloroform-methanol eluant gradiently,then the compounds were isolated by using ODS column and silica gel thin-layer preparation plate,and the effects of the compounds on the growth of human umbilical vein vascular endothelial cells were determined by MTT assay.RESULTS:A total of three compounds were isolated.The compounds could promote or inhibit the growth of human umbilical vein vascular endothelial cells.CONCLUSION:The established method is suitable for the separation and purification of the hydrophilic components of the ethanol extract of P.multiflorum.It is initially found out that the compounds that can promote the growth of human umbilical vein vascular endothelial cell were contained in P.multiflorum.
