Determination of Cinobufagin and Resibufogenin in Yigankang Capsule by HPLC
- VernacularTitle:HPLC法测定益肝康胶囊中华蟾酥毒基与脂蟾毒配基的含量
- Author:
Xiaoying DUAN
;
Qiang LI
- Publication Type:Journal Article
- Keywords:
Yigankang capsule;
Cinobufagin;
Resibufogenin;
HPLC;
Content determination
- From:
China Pharmacy
2007;0(30):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of the contents of cinobufagin and resibufogenin in Yigankang capsule.METHODS:The samples were separated on Spherigel ODS C18(150 mm?4.6 mm,5 ?m)chromatographic column with 0.5% KH2PO4-acetonitrile(50∶50,pH was adjusted to 3.2 by phosphoric acid)used as a mobile phase at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 296 nm and the column temperature was kept at 40 ℃.RESULTS:The linear ranges of cinobufagin and resibufogenin were 0.22～1.09 ?g(r=0.999 8)and 0.26～1.30 ?g(r=0.999 9),respectively,and the average recoveries of cinobufagin and resibufogenin were 99.4%(RSD=1.9%,n=9)and 98.7%(RSD=1.4%,n=9),respectively.CONCLUSION:This method is proved to be sensitive,simple,accurate and specific,and it is applicable for the quality control of Yigankang capsule.
