Determination of Theophylline Concentration in Human Plasma by Micellar Electrokinetic Capillary Chromatography
- VernacularTitle:胶束电动毛细管色谱法测定人血浆中茶碱的浓度
- Author:
Wenyue ZHANG
;
Suodi ZHAI
- Publication Type:Journal Article
- Keywords:
Micellar electrokinetic capillary chromatography;
Theophylline;
Plasma drug concentration;
Therapeutic drug monitoring
- From:
China Pharmacy
2007;0(29):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a simple micellar electrokinetic capillary chromatography for determination of theophylline concentration in human plasma. METHODS: The determination of plasma sample(without any pretreatment) was performed on un-coated fused-silica capillaries (at an effective length of 21 cm) in which the cathodic buffer solution (solution A) was 15 mmol?L-1 borax(pH=10) and the running buffer solution(solution B) was sodium dodecyl sufate (SDS)(200 mmol?L-1) -contained solution A. The sample was injected into capillary by pressure with separation voltage of 12 kV;the UV detection wavelength was set at 280 nm;quantitation was performed by external standard peak area method. RESULTS: The average determination time of each sample was 11 min;the linear range of theophylline was 1.25～40 ?g?mL-1 with its lowest detectable limit at 0.5 ?g?mL-1;the average methodological recovery was 90.33%(RSD=2.51%);the intra-day RSD was 2.34%～3.36% and the inter-day RSD was 2.03%～6.51%,respectively. CONCLUSION: The developed method is simple,rapid and sensitive and it is applicable for the therapeutic drug monitoring of theophylline.
