RAM-HPLC vs.FPIA for Determination of Plasma Concentration of Carbamazepine
- VernacularTitle:限进介质-HPLC法与荧光偏振免疫法测定人血浆中卡马西平浓度的比较
- Author:
Qian XU
- Publication Type:Journal Article
- Keywords:
Carbamazepine;
Restricted-access media;
HPLC;
FPIA
- From:
China Pharmacy
2007;0(29):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a RAM(Restricted-access media)-HPLC for the determination of plasma concentration of carbamazepine,and to compare the determination results with those determined by FPIA method. METHODS: The plasma samples were centrifugalized,then the venous plasma samples were determined by FPIA and RAM-HPLC,respectively,while the fingertip plasma sample was determined by RAM-HPLC alone. The chromatographic column was Hisep shielded hydrophobic phase analytical column,and the mobile phase consisted of ammonium acetate buffer-acetonitrile (88∶12) at a flow rate of 1.5 mL?min-1. The detective wavelength was set at 280 nm;the sample size was 20 ?L and the column temperature was kept at room temperature. RESULTS: A good correlation was noted between the carbamazepine concentration in venous plasma sample determined by FPIA and carbamazepine concentrations in either venous or fingertip plasma determined by RAM-HPLC,with the correlation coefficients being 0.989 and 0.995,respectively,but the differences were significant(P0.05). CONCLUSION: Both RAM-HPLC and FPIA are applicable for the determination of carbamazepine concentration,but FPIA is more suitable for routine monitoring while RAM-HPLC is more suitable for the related research and monitoring of specific cases. Both methods shows good reproducibility and in which the samples don't have to be pretreated.
