Role of danshensu on TGF-? signal transduction in rat's hepatic stellate cells
- VernacularTitle:丹参素对肝星状细胞TGF-?信号转导的影响
- Author:
Qing DAI
;
Xin LI
;
Lei ZHENG
;
Zhi DONG
- Publication Type:Journal Article
- Keywords:
Hepatic stellate cells;
Danshensu;
Transforming growth factor beta;
Receptors,transforming growth factor;
Smads pathway
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the role of danshensu on Smad signal transduction in rat hepatic stellate cells(HSCs)stimulated with transforming growth factor(TGF-?1).METHODS:The rat HSCs was isolated with collagenase by in situ-liver recirculation perfusion and cultured in vitro.MTT colorimetric assay was used to detect proliferation of HSCs treated with different concentration of danshensu.The expressions of ?-SMA and T?R I and II were observed by immunocytochemitry,indirect immunofluorescent staining and Western blotting when HSCs stimulated with TGF-?1 and with different concentrations of danshensu for 24 h.RESULTS:(1)Danshensu at the concentration from 0.0625 mmol/L to 1 mmol/L prevented the proliferation of HSCs in a dose-dependent manner(P
