Apoptotic mechanism of neuron-like cells differentiated from adult rat mesenchymal stem cells in vitro
- VernacularTitle:大鼠MSCs体外诱导分化为神经元样细胞过程中发生细胞凋亡的机制研究
- Author:
Tao HE
;
Li WANG
;
Xiaoyan LIU
;
Ning FENG
;
Juan LI
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Neurons;
Apoptosis;
Signal transduction
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the mechanism of apoptosis during the process of adult rat mesenchymal stem cells(MSCs)differentiating into neuron-like cells in vitro.METHODS:The MSCs were isolated primarily from adult rats bone marrow by density gradient and then expanded in medium as undifferentiated cells for 3-5 generations.The MSCs were divided into three groups at random.The control group was induced with ?-mercaptoethanol(?-ME).Meanwhile,the U0126 group was given ?-ME and U0126(10 ?mol/L)added at the beginning of induction.The PMA groups were treated with ?-ME and different concentrations of PMA since pre-induction.The effects of U0126 and PMA on the activity of neuron-like cells were observed by MTT.The effects of U0126 and PMA on the expression of neuron specific marker nestin and expression of apoptosis-related protein caspase,Bcl-2,Bax in neuron-like cells were detected by using immunocytochemistry method.TUNEL technique was used to detect apoptosis index.RESULTS:Compared to control group,neuron viability,nestin and Bcl-2 increased and neuron apoptosis decreased in U0126 group(P
