Effect of glucocorticoid-treated dendritic cells on asthmatic Th2 response
- VernacularTitle:激素预处理树突状细胞对哮喘Th2型反应的影响
- Author:
Hongtao LI
;
Jin HUANG
;
Tiantuo ZHANG
- Publication Type:Journal Article
- Keywords:
Asthma;
Dendritic cells;
Dexamethasone;
Cytokines
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of dexamethasone-treated dendritic cells(DCs)on Th2 cytokine production from autologous T cells in asthmatic patients and explore the mechanisms by studying the effect of dexamethasone on differentiation,maturation and function of DCs from patients with asthma.METHODS:Human peripheral blood monocyte-derived DCs generated from asthmatic patients and healthy subjects were cultured in the absence or presence of dexamethasone.The phenotypic characterization of DCs was analyzed by flow cytometry.The mature DCs were harvested,washed,and then cocultured in vitro with autologous T cells purified by a nylon cotton column.The DC-T coculture supernatants were collected after 72 h incubation and analyzed for levels of IL-5 and IFN-? by ELISA.RESULTS:The concentrations of IL-5 in the culture supernatants of DC-T coculture were significantly up-regulated in patients with asthma compared with that in healthy controls [(145.13?89.76)ng/L vs(50.28?22.37)ng/L,P0.05)].There were significantly decreased levels of IL-5 by autologous T cells primed by dexamethasone-treated mature DCs from asthmatic patients [(45.39?19.61)ng/L vs(145.13?89.76)ng/L,P0.05).IFN-? production was decreased by autologous T cells primed by dexamethasone-treated mature DCs from both asthmatic patients and healthy controls [asthma group:(40.21?22.89)ng/L vs(197.58?76.32)ng/L,P
