The effect and mechanism of wortmannin on human gastric carcinoma cells
- VernacularTitle:渥曼青霉素对人胃癌细胞的作用及机制
- Author:
Long QIN
;
Caiquan ZHANG
- Publication Type:Journal Article
- Keywords:
Stomach Neoplasms;
NF-?B;
Wortmannin;
Protein Kinase B
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of wortmannin on the human gastric carcinoma cells. Methods SGC7901 cells were treated with 10nmol/L,30nmol/L and 60nmol/L of wortmannin,a specific inhibitor of AKT,for different time periods. Cell viability was estimated by MTT assay. Western blot was used to detect the level of NF-?B neucleoprotein and reverse transcriptasepolymerase chain reaction(RT-PCR) was used to determine transcription of NF-?B mRNA. Results All three different concentrations of wortmannin could inhibit the growth of SGC7901 cells,and the depression effect obviously depended on time and drug dose(P0.05). As the action time of wortmannin was prolonged,neucleoprotein and mRNA expression of NF-?B significantly decreased compared with the control (P
