Effect of Arsenic Trioxide on Human Hepatoma Cell Proliferation and Its uPAR Expression
- VernacularTitle:三氧化二砷对人肝癌细胞生长及其尿型纤溶酶受体表达的体外研究
- Author:
Yan ZHOU
;
Jiawei YU
;
Hongxia WU
;
Zhijian YU
- Publication Type:Journal Article
- Keywords:
Arsenic trioxide;
Hepatoma cell SMMC-7721;
Proliferation;
uPAR;
Expression
- From:
China Pharmacy
2007;0(28):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of arsenic trioxide(As2O3)on human hepatoma cells SMMC-7721 viability and the expression of urokinase plasminogen activator receptor(uPAR).METHODS:SMMC-7721 cells were cultured and treated with As2O3 at different concentrations for different time with untreated SMMC-7721 cells(without As2O3 treatment)served as control.The proliferation rate of the SMMC-7721 cells was measured by MTT assay;the change of cell cycle was detected by flow cytometry analysis;the effect of As2O3 on uPAR mRNA levels was analyzed by real-time fluorescent quantitative polymerase chain reaction.RESULTS:As2O3 had significant inhibitory effect on SMMC-7721 proliferation in time-dependent and concentration-dependent manner.As compared with control group,As2O3 significantly decreased the proportion of cells in G0/G1 phase but increased the cell proportion in G2/M phase,and it markedly down-regulated the level of uPAR mRNA expression(P
