Determination of the Entrapment Efficiency of Tetracaine Hydrochloride Liposome by the Microcolumn Centrifugation-HPLC
- VernacularTitle:微柱离心-HPLC法测定盐酸丁卡因脂质体包封率
- Author:
Min WANG
;
Yingjie DENG
;
Na WANG
- Publication Type:Journal Article
- Keywords:
Tetracaine hydrochloride liposome;
Entrapment efficiency;
Microcolumn centrifugation;
HPLC
- From:
China Pharmacy
2007;0(28):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a method for determining the entrapment efficiency of tetracaine hydrochloride liposome.METHODS:The microcolumn centrifugation was adopted to separate the free tetracaine hydrochloride from liposomes;the content of tetracaine hydrochloride was determined by HPLC and the entrapment efficiency was computed.RESULTS:By microcolumn centrifugation,the liposomes and the free tetracaine hydrochloride were well-separated.The recovery rate of the blank liposomes was 90.4%～100.1%;the absorption rate of free tetracaine hydrochloride was 96.6%～99.2%;and the entrapment efficiencies of tetracaine liposomes stood at about 80%.CONCLUSION:This method established for determining the entrapment efficiency of tetracaine hydrochloride liposome is simple,rapid and reproducible.
