Determination of 10-deacetyl-bacratin Ⅲ in Taxus baccata by RP-HPLC
- VernacularTitle:RP-HPLC法测定欧洲红豆杉中10-脱乙酰巴卡亭Ⅲ的含量
- Author:
Hua ZHEN
- Publication Type:Journal Article
- Keywords:
Taxus baccata;
10-deacetyl-bacratin Ⅲ;
RP-HPLC;
Content determination
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the RP-HPLC method for determination of the content of 10-deacetyl-bacratin Ⅲ in Taxus baccata.METHODS:The determination was performed on Shim-Pack VP-ODS column (250 mm?4.6 mm,5 ?m) at a column temperature of 40 ℃.The mobile phase consisted of acetonitrile-water (gradient elution) at a flow rate of 1.0 mL?min-1.The detective wavelength was set at 232 nm. RESULTS:The linear range of 10-deacetyl-bacratin Ⅲ was 264～1 320 ng(r=0.999 5)and the average recovery was 98.8%(RSD=1.3%,n=9).CONCLUSION:The method is simple,accurate and reproducible,and it is applicable for the quality control of T.baccata.
