The Effect of Erythropoietin on the Production of Endothelin in Human Glomerular and Umbilical Endothelial Cells.
- Author:
Su Kil PARK
1
;
Seong Who KIM
;
Won Seok YANG
;
Chung Soo KIM
;
Han Jong AHN
;
Jung Sik PARK
;
Jae Dam LEE
Author Information
1. College of Medicine, University of Ulsan Department of Internal Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Erythropoietin;
endothelin;
Glomerular endothelial cells, Human umbilical endothelial cells;
TGF-beta;
TNF-alpha;
Thrombin
- MeSH:
Arterial Pressure;
Endothelial Cells*;
Endothelins*;
Enzyme-Linked Immunosorbent Assay;
Erythrocytes;
Erythropoietin*;
Human Umbilical Vein Endothelial Cells;
Humans*;
Hypertension;
Muscle, Smooth, Vascular;
Renal Insufficiency;
Thrombin;
Transforming Growth Factor beta;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Nephrology
1998;17(3):383-390
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The administration of erythropoietin for renal failure patients is frequently associated with a mild-to-marked rise in arterial blood pressure. The erythropoietin-induced hypertension has been variably attributed to the rise in erythrocyte concentration and/or a direct or indirect pressor action of erythropoietin on vascular smooth muscle. Especially erythropoietin-induced hypertension may be mediated by increased production of the potent vasoactive peptide endothelin. This study was designed to determine the effect of erythropoietin to the production of endothelin in human glomerular endothelial cells and human umbilical vein endothelial cells. ELISA method was used to measure the endothelin- 1, after 4 X 10(4) endothelial cells were grown to confluency on 24-well plates, in which erythropoietin 25u/ml was incubated for 24 hours. The results showed that the endothelin production in human glomerular endothelial cells was insignificant (116.0+/-14.0%, P>0.05, mean+/-S.E., n=5, each n means the mean of duplicate experiments), but the endothelin production in human umbilical endothelial cells was increased after 25u/ml erythropoietin treatment(126.5+/-15.2%, P<0.05, mean+/-S.E., n=4). In glomerular endothelial cells, TGF-beta(0.5ng/ml) increased the production of endothelin(218.8+/-57.2%, P<0.01, mean+/-S.E., n=5), also it looks like that TNF-alpha and thrombin might increase the production of endothelin according to the concentration. In conclusion, the responsiveness of endothelial cells to erythropoietin may be different according to the cell type, and glomerular endothelial cells could increase the production of endothelin, if appropriate stimuli were given.
