Determination of the Content of Vitamin A Palmitate and Its Related Substances by HPLC
- VernacularTitle:HPLC法测定维生素A棕榈酸酯原料药中主成分及有关物质含量
- Author:
Ai LIU
;
Xiaohai TANG
;
Yongmei XIE
;
Xiaofang HUO
;
Dan YANG
;
Xiao HU
;
Hang SONG
- Publication Type:Journal Article
- Keywords:
HPLC;
Vitamin A palmitate;
Related substance;
Content
- From:
China Pharmacy
2007;0(25):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establishment the method for the determination of content of vitamin A palmitate(VAP) and its related substances by HPLC. METHODS:The HPLC conditions were consisted of Phenomenex Luna C18 column with a mobile phase of a mixture of acetonitrile-isopropanol (90∶10) ,the detection wavelength of 328 nm,the column temperature of 30 ℃ and the flow rate of 1.0 mL?min-1. RESULTS:VAP was completely separated from impurities,the linearity range was 90～400 mg?L-1(r=0.999 2). The average recovery rate was 99.60% (RSD=1.32%). The average content of the related substances were lower than 2.53% . CONCLUSION: This accurate and reliable HPLC method is applicable for the quality control of VAP.
